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Handbook of Optical Biomedical Diagnostic: Methods presenting the basic principles and diagnostic applications of optical techniques. Detecting and processing the scattering, fluorescence, FT IR, and Raman spectroscopic signals from various tissues, with an emphasis on blood, epithelial tissues, and human skin. The second half of the volume discusses specific imaging technologies, such as Doppler, laser speckle, optical coherence tomography (OCT), and fluorescence and photoacoustic imaging.
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- Part I describes the fundamentals and basic research of the extinction of light in dispersive media; the structure and models of tissues, cells, and cell ensembles; blood optics; coherence phenomena and statistical properties of scattered light; and the propagation of optical pulses and photon-density waves in turbid media. Tissue phantoms as tools for tissue study and calibration of measurements are also discussed.
- Part II presents time-resolved (pulse and frequency-domain) imaging and spectroscopy methods and techniques applied to tissues, including optoacoustic (photoacoustic) methods. The absolute quantification of the main absorbers in tissue by a NIR spectroscopy method is discussed. An example biomedical application – the possibility of monitoring brain activity with NIR spectroscopy – is analyzed.
- Part III presents various spectroscopic techniques of tissues based on elastic and Raman light scattering, Fourier transforms infrared (FTIR), and fluorescence spectroscopies. In particular, the principles and applications of backscattering diagnostics of red blood cell (RBC) aggregation in whole blood samples and epithelial tissues are discussed. Other topics include combined back reflectance and fluorescence, FTIR and Raman spectroscopies of the human skin in vivo, and fluorescence technologies for biomedical diagnostics.
- The final section, Part IV, begins with a chapter on laser Doppler microscopy, one of the representative coherent-domain methods applied to monitor blood in motion. Methods and techniques of real-time imaging of tissue ultrastructure and blood flow using OCT are also discussed. The section also describes various speckle techniques for monitoring and imaging tissue, in particular, for studying tissue mechanics and blood and lymph flow.
Financial support from a FiDiPro grant of TEKES, Finland (40111/11) and Academic D.I. Mendeleev Fund Program of Tomsk National Research State University has helped me complete this book project. I greatly appreciate the cooperation and contribution of all of the authors and co-editors, who have done great work on the preparation of this book. I would like to express my gratitude to Eric Pepper and Tim Lamkins for their suggestion to prepare the second edition of the Handbook and to Scott McNeill for assistance in editing the manuscript. I am very thankful to all of my colleagues from the Chair and Research Education Institute of Optics and Biophotonics at Saratov National Research State University and the Institute of Precision Mechanics and Control of RAS for their collaboration, fruitful discussions, and valuable comments. I am very grateful to my wife and entire family for their exceptional patience and understanding.
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